When the optical density at 600 nm (OD600nm) of the candida reached 2C5, the candida cells were collected by centrifugation (1000 = 5)
When the optical density at 600 nm (OD600nm) of the candida reached 2C5, the candida cells were collected by centrifugation (1000 = 5). were expressed using surface display technology. The yeast-prepared oral vaccines were named EBY100-OMCP and EBY100-LTB-OMCP. The candidate vaccines could resist the acidic intestinal environment. After 7 days of continuous oral immunization, signals of innate and adaptive immunity were measured on days 1, 7, 14, 21, 28, 35, and 42. Large activities of immune enzymes (T-SOD, AKP, ACP, and LZM) in serum and intestinal mucus were detected. in the head kidney was significantly upregulated (EBY100-OMCP group: 3.8-fold; BY100-LTB-OMCP group: 4.3-fold). was upregulated in the intestines (EBY100-OMCP group: 5.6-fold; EBY100-LTB-OMCP group: 6.7-fold). Serum Rabbit Polyclonal to HNRNPUL2 neutralizing antibody titers of the two organizations reached 1:85. Dental vaccination safeguarded…