Boca Raton, Fla: CRC Press; 1994
Boca Raton, Fla: CRC Press; 1994. elicited by gp43, which may halt the progression of the illness, along with a locally effective but generally unprotective antibody response to this antigen, we aimed in the present investigation at determining the epitopes mediating these immune responses and at selecting those that could become used in vaccination tests. We show here that a 15-amino-acid peptide (P10) contained in gp43 is responsible for glycoprotein-mediated T-cell activation and safety against PCM in BALB/c mice without eliciting antibodies directed against the native antigen. MATERIALS AND METHODS Purification of gp43 from B-339, originally obtained from A. Restrepo-Moreno, Medellin, Colombia, was cultivated in candida extract-peptone-dextrose (YPD) liquid medium for 7 days at 36C with shaking. The tradition was killed by adding 0.2 g of merthiolate/liter filtered through a…