Among them, P10A7 and P5C7 also showed strong or moderate functional cross-reactivity to the heterologous CS17+ ETEC strain WS6788A, suggesting the three residues (R67, S86, and R181) identified by P10A7 and P5C7 were functional epitope residues shared between CfaE and CsbD
Among them, P10A7 and P5C7 also showed strong or moderate functional cross-reactivity to the heterologous CS17+ ETEC strain WS6788A, suggesting the three residues (R67, S86, and R181) identified by P10A7 and P5C7 were functional epitope residues shared between CfaE and CsbD. a fimbrial tip adhesin. However, sequence variability among the class 5 adhesins may Cinnamic acid hinder the generation of cross-protective antibodies. To better understand practical epitopes of the class 5 adhesins and their ability to induce intraclass antibody reactions, we produced 28 antiadhesin monoclonal antibodies (MAbs) to representative adhesins CfaE, CsbD, and CotD, respectively. We identified the MAb cross-reactivities, localized the epitopes, and measured functional activities as potency in inhibition of hemagglutination induced by class 5 fimbria-bearing ETEC. The MAbs reactivities to a panel of class 5 adhesins in…