Pellets were resuspended in MiliQ H2O and Tricine buffer in a ratio 1:2
Pellets were resuspended in MiliQ H2O and Tricine buffer in a ratio 1:2. was then subjected to HPLC and the effluent fed to an ESI-TOF detector. The inset shows the total ion current LY2801653 dihydrochloride output. The MS spectrum of the peak in at 18.9C19.6 minutes is shown. Deconvolution of the spectrum yielded a main component with an average mass of 9400.76 Da (MH+), corresponding to a peptide with the Mo PrP sequence M153-S230 (theoretical average mass: 9399.5 Da). AN Additional species with an CTSD MH+ value of +17 LY2801653 dihydrochloride and likely corresponds to the same peptide with one of the three Met residues present in the sequence oxidized. A minor component with an MH+ value of 9531.61 Da might correspond to N152-S230 (theoretical average mass: 9513.6) with an…