(f) the eluate from E was then purified using anion-exchange chromatography; the eluate (purified Haidalimumab) was collected
(f) the eluate from E was then purified using anion-exchange chromatography; the eluate (purified Haidalimumab) was collected. Abbreviations: ELISAenzyme linked immunosorbent assayRArheumatoid arthritisSDS-PAGEsodium dodecyl sulfate polyacrylamide gel electrophoresisrhTNFrecombinant human tumor necrosis factor-alphaEC50concentration for 50% of maximal effectTNF-Tg micetumor necrosis factor transgenic miceAMDactinomycin DMTTmethylthiazolyldiphenyl-tetrazolium bromidePBSphosphate\buffered saline and conjugated to polyethylene glycolDNA recombinant technology in CHO cellsDNA recombinant technology in CHO cells Open in a separate window TNF: tumor necrosis factor, sTNF: soluble TNF, tmTNF: transmembrane TNF, Licogliflozin LT-: lymphotoxin-. This study was aimed at researching and developing TNF antibodies with low immunogenicity and with functions similar to that of human antibodies. We isolated and screened B cells secreting anti-TNF antibody from rheumatoid arthritis patients. We cloned the heavy chain and the light chain sequences of anti-TNF antibody and Licogliflozin constructed a…