{"id":505,"date":"2023-04-01T05:01:20","date_gmt":"2023-04-01T05:01:20","guid":{"rendered":"http:\/\/changingfaceofamerica.com\/?p=505"},"modified":"2023-04-01T05:01:20","modified_gmt":"2023-04-01T05:01:20","slug":"note-the-desmin-signal-was-detected-at-the-periphery-of-the-myoblast-but-not-observed-in-the-fibroblast","status":"publish","type":"post","link":"https:\/\/changingfaceofamerica.com\/?p=505","title":{"rendered":"\ufeffNote the desmin signal was detected at the periphery of the myoblast but not observed in the fibroblast"},"content":{"rendered":"

\ufeffNote the desmin signal was detected at the periphery of the myoblast but not observed in the fibroblast. Z-axis of chick myogenic cells showing the differences in the distribution of microtubules, and intermediate filament desmin and nuclei between finger-shaped fibroblasts and mushroom-like muscle cells after 72 hrs of cytochalasin B treatment. The 3-m apart images were taken from a stack of 1-m interval slices, acquired UAA crosslinker 2<\/a> using a laser scanning confocal microscope. Cells were labeled with antibodies against -tubulin (green) and desmin (red), and with DAPI (blue). Compare the spread and flat fibroblasts with the round and thick desmin-positive myoblasts. Note that the fibroblast nuclei are located in the 6 and 3-m slices whereas the myoblast nuclei are UAA crosslinker 2 present in all the slices. Scale bar, 10 m.(TIF) pone.0154109.s003.tif (4.5M) GUID:?47D75AFE-AE27-4DD2-8D70-9BFC8E0EC445 S4 Fig: 3D reconstruction of chick myogenic cells showing distinct morphological differences between a finger-shaped fibroblast and a mushroom-like muscle cell after 72 hrs of cytochalasin B treatment. Sequential projections in the X and Y-axis from a stack of 1-m interval images, acquired using a laser scanning confocal microscope. Cells were labeled with antibodies against -tubulin (green) and desmin (red), and with DAPI (blue). Note the desmin signal was detected at the periphery of the myoblast but not observed in the fibroblast. Microtubules are well spread in the fibroblast and concentrated in the center of the round myoblast. Both cells are binucleated. Scale bar, 10 m.(AVI) pone.0154109.s004.avi (3.4M) GUID:?93766644-D6FD-4B80-827D-17CF41ED7C3C S5 Fig: Selected X-Z and Y-Z sections and selected slice of a confocal stack showing a finger-shaped fibroblast after 72 hrs of cytochalasin B treatment. The sections were reconstructed from a stack of 22 images with 1-m interval, acquired using a laser scanning confocal microscope. Cells were labeled with Rho-phalloidin (red), an antibody against -tubulin (green), and DAPI (blue). Note that actin accumulates at the tip of the fibroblast fingers (close to the substrate), whereas microtubules (that label most of the cell cortex) do not localize to the very extremity of the cell. Scale bar, 5 m.(TIF) pone.0154109.s005.tif (1023K) GUID:?875F5625-907D-4231-99A2-FFC7595FDE68 S6 Fig: 3D reconstruction of a muscle cell after 72 hrs of cytochalasin B treatment. Sequential projections in the UAA crosslinker 2 X and Y-axis from a stack of 1.4-m interval images, acquired using a laser scanning confocal microscope. Cells were labeled with an antibody against sarcomeric–actinin (red) and DAPI (blue). Note that the round myoblast contains perfectly striated myofibrils, as seen in the Z-line staining of sarcomeric–actinin. The round myoblast has UAA crosslinker 2 4 nuclei. Scale bar, 5 m.(AVI) pone.0154109.s006.avi (2.4M) GUID:?64947696-57D9-48B7-89C7-562F7F1A3A58 S7 Fig: Timelapse movie of cell shape changes during recovery of muscle cells and fibroblasts from long term-CB treatment. During the initial 20 min of recovery after 72 hrs of CB treatment, myogenic cells remain globular shaped whereas finger-shaped fibroblasts loses their finger protrusions and changes into flatten multinucleated spread cells. Time is shown in the top left of each image. The movie was taken from the region shown in Fig 8D inset. Scale bar, 10 m.(AVI) pone.0154109.s007.avi (3.5M) GUID:?AF4A0F6C-D031-46D8-86BF-F389A968A106 S8 Fig: DMSO shows no effects on myogenesis after long-term treatment of chick myogenic cell cultures. Phase contrast microscopy images of chick myogenic cells treated on day 2 with shows DMSO for 3 days are shown (A-C). Note that cultures treated with DMSO are indistinguishable from control-untreated cultures (compare A and B). In both conditions (control-untreated and DMSO-treated cultures) it is possible to see the formation of long multinucleated myotubes (A and B). Cultures treated with CB for 3 days show finger-shaped fibroblastic cells (indicated by white arrow in C) Sdc2<\/a> and globular-shaped myoblasts (indicated by black arrow in C). Scale bar, 20 m.(TIF) pone.0154109.s008.tif (2.0M) GUID:?24E97748-1A65-47C0-B1D8-C7AF8B060354 S9 Fig: Sequential images in the Z-axis of a chick myotube showing the different.<\/p>\n","protected":false},"excerpt":{"rendered":"

\ufeffNote the desmin signal was detected at the periphery of the myoblast but not observed in the fibroblast. Z-axis of chick myogenic cells showing the differences in the distribution of microtubules, and intermediate filament desmin and nuclei between finger-shaped fibroblasts and mushroom-like muscle cells after 72 hrs of cytochalasin B treatment. The 3-m apart images […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[33],"tags":[],"class_list":["post-505","post","type-post","status-publish","format-standard","hentry","category-cell-signaling"],"_links":{"self":[{"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=\/wp\/v2\/posts\/505","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=505"}],"version-history":[{"count":1,"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=\/wp\/v2\/posts\/505\/revisions"}],"predecessor-version":[{"id":506,"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=\/wp\/v2\/posts\/505\/revisions\/506"}],"wp:attachment":[{"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=505"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=505"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/changingfaceofamerica.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}