5 B)
5 B). and calcium mineral influx. Signaling induced by phorbol myristate acetate (PMA) and ionomycin had not been significantly reduced, recommending SLAP-2 features in the antigen receptor signaling cascade proximally. The SLAP-2 proteins consists of an NH2-terminal myristoylation consensus SH3 and series and SH2 Src homology domains, but does not have a tyrosine kinase site. In antigen receptorCstimulated cells, SLAP-2 connected with many tyrosine phosphorylated proteins, like the ubiquitin ligase Cbl. Deletion from the COOH terminus of SLAP-2 clogged function and abrogated its association with Cbl. Mutation from the putative myristoylation site of SLAP-2 jeopardized its inhibitory activity and impaired its localization towards the membrane area. Our identification from the adverse regulator SLAP-2 demonstrates a retroviral-based testing strategy could be an efficient method to recognize and characterize the function of…