McNiven, L
McNiven, L. as well as the role of its CT. We found that CD1a can be internalized via a clathrin- and dynamin-independent pathway and that it follows a Rab22a- and ARF6-dependent recycling pathway, similarly to other cargo internalized independently of clathrin. We also found that the CD1a CT is S-acylated. However, this Ac-IEPD-AFC post-translational modification does not determine the rate of internalization or recycling of Ac-IEPD-AFC the protein, or its localization to detergent-resistant membrane microdomains (DRM), where we found CD1a to be enriched. We also show that plasma membrane DRM are essential for efficient CD1a-mediated antigen presentation. These findings place CD1a closer to MHC class I in its trafficking and potential antigen-loading compartments, among CD1 isoforms. Furthermore, we identify CD1a as Ac-IEPD-AFC a new marker for the clathrin- and dynamin-independent…