The upper a part of panel C shows representative Western blots using anti-pAkt, anti-p-ERK1/2 or anti-Tubilin antibodies and each antibody was used in different membranes
The upper a part of panel C shows representative Western blots using anti-pAkt, anti-p-ERK1/2 or anti-Tubilin antibodies and each antibody was used in different membranes. phosphorylation whereas DHA preserved insulin action. Palmitic acid up-regulated TLR4 as well as pro-inflammatory cytokines IL6 and TNF contrasting with DHA effect. Similarly to palmitic acid, resistin treatment induced the up-regulation of IL6 and TNF as well as NFB activation. Importantly, palmitic acid potentiated the resistin-dependent NFkB activation whereas DHA abolished it. The recruitment of TLR4 to membrane lipid rafts was increased by palmitic acid treatment; this is concomitant with the augmentation of resistin-induced TLR4/MYD88/TIRAP complex formation required for TLR4 signaling. In conclusion, palmitic acid increased TLR4 expression promoting resistin signaling through TLR4 up-regulation and its recruitment to membrane lipid rafts. 106 cells per well…